TrueBlack® Lipofuscin Autofluorescence Quencher, 20X in DMF from Biotium, Inc.

Product Specs
ItemTrueBlack® Lipofuscin Autofluorescence Quencher, 20X in DMF
CompanyBiotium, Inc.
Price Supplier Page View Company Product Page
Catalog Number23007
Quantity1 x 1ML

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Biotium, Inc.

46117 Landing Parkway

Fremont, CA 94538

United States

Phone: 510-265-1027
510-265-1352

Email: order@biotium.com

Company Profile

Website: https://biotium.com/company/

(1) Quantifying Cell Proliferation Through Immunofluorescence on Whole-Mount and Cryosectioned Regenerating Caudal Fins in African KillifishBio ProtocDecember 20, 2023Augusto Ortega Granillo, Robert R. Schnittker, Wei Wang, Alejandro Sánchez Alvarado(OCT) embedding compound (SAKURA, catalog number: 4583)TrueBlack lipofuscin (Biotium, catalog number: 23007)ProLong Gold antifade mountant (Thermo Fisher Scientific, catalog number: P36930)Tsugar memory miniature

(2) Protocol for multispectral imaging on cryosections to map myeloid cell heterogeneity in its spatial contextSTAR protocolsDecember 15, 2023Wieland EB, Kempen LJAP, Lu C, Donners MMPC, Biessen EAL, Goossens P.VWRK4085.9001 Eosin,VWR/Q Path Chemicals,Cat# 10047101 TrueBlack lipofuscin autofluorescence quencher,Biotium,Cat# 23007 Sudan Black B,Sigma-Aldrich,Cat# 199664 ,, Software and algorithms,Software and algorithms,Software

(3) Severe central nervous system demyelination in Sanfilippo diseaseFront Mol NeurosciDecember 13, 2023Mahsa Taherzadeh, Erjun Zhang, Irene Londono, Benjamin De Leener, Sophie Wang, Jonathan D. Cooper, Timothy E. Kennedy, Carlos R. Morales, Zesheng Chen, Gregory A. Lodygensky, et al.sections were dipped briefly in TrueBlack® Lipofuscin Autofluorescence Quencher (dilution 1:10, Biotium, 23007) for 1 min, and then washed with PBS. The slides were mounted with Prolong Gold Antifade mounting reagent

(4) Immunolocalization of Sphingolipid Catabolism Enzymes along the Nephron: Novel Early Urinary Biomarkers of Renal DamageInternational Journal of Molecular SciencesNovember 23, 2023Martha Franco, Agustina Cano-Martínez, María del Pilar Ramos-Godínez, Rebeca López-Marure, Luis Donis-Maturano, José Santamaría Sosa, Rocio Bautista-Pérezprimary antibody and to reduce autofluorescence, we used TrueBlack Lipofuscin Autofluorescence Quencher (#23007; Biotium, TermoFisher, Waltham, MA, USA) [62]. Fluorescence images were obtained using a Cell Imaging

(5) Loss of TMEM106B exacerbates Tau pathology and neurodegeneration in PS19 micebioRxivNovember 18, 2023Tuancheng Feng, Huan Du, Fenghua Hu(Electron Microscopy Sciences, 62550–01), TrueBlack Lipofuscin Autofluorescence Quencher (Biotium, 23007), Odyssey blocking buffer (LI-COR Biosciences, 927–40000), and Fluoromount-G (Thermo scientific, E113391).

(6) Dissecting the human leptomeninges at single-cell resolutionNature CommunicationsNovember 3, 2023Nicola A. Kearns, Artemis Iatrou, Daniel J. Flood, Sashini De Tissera, Zachary M. Mullaney, Jishu Xu, Chris Gaiteri, David A. Bennett, Yanling WangAutofluorescence was quenched with a 30-second exposure to True-Black Lipofuscin Autofluorescence Quencher (23007, Biotium) and washed with PBS before mounting. Imaging was performed on either a CellInsight CX7 LZR

(7) A marmoset brain cell census reveals regional specialization of cellular identitiesScience AdvancesOctober 13, 2023Fenna M. Krienen, Kirsten M. Levandowski, Heather Zaniewski, Ricardo C.H. del Rosario, Margaret E. Schroeder, Melissa Goldman, Martin Wienisch, Alyssa Lutservitz, Victoria F. Beja-Glasser, Cindy Chen, et al.or ACD antisense probes and incubated with TrueBlack Lipofuscin Autofluorescence Quencher (Biotium, 23007) for 10 s at room temperature to eliminate confounding lipofuscin autofluorescence present in the tissue.

(8) A New Role for RNA G-quadruplexes in Aging and Alzheimer’s DiseasebioRxivOctober 10, 2023Lena Kallweit, Eric D. Hamlett, Hannah Saternos, Anah Gilmore, Ann-Charlotte Granholm, Scott Horowitzrinsed again with TBS and incubated with 1X True Black Lipofuscin Autofluorescence Quencher (Biotium CAT#23007) diluted in 70% ethanol. Slides were washed 3 times with TBS then incubated with primary antibodies

(9) Tracking cell-type-specific temporal dynamics in human and mouse brainsCellSeptember 28, 2023Lu Z, Zhang M, Lee J, Sziraki A, Anderson S, Zhang Z, Xu Z, Jiang W, Ge S, Nelson PT, et al.USA). Sections were next incubated for 45 s at room temperature in a 1X solution of TrueBlack (Cat # 23007, Biotium, Fremont, CA) prepared in 70% ethanol, to reduce auto-fluorescence. Sections were blocked in

(10) Creation of a decellularized vaginal matrix from healthy human vaginal tissue for potential vagina reconstruction – experimental studiesInternational Journal of SurgerySeptember 27, 2023Jayson Sueters, Fangxin Xiao, Jan-Paul Roovers, Mark-Bram Bouman, Freek Groenman, Huub Maas, Judith Huirne, Theo SmitFibronectin-slides, quenching of auto fluorescence was performed by 30 s incubation with 1× TrueBlack (#23007; Biotium, Fremont, California, USA) diluted in 70% ethanol, followed by a 5 min washing step in PBS

(11) In-depth comparison of Anc80L65 and AAV9 retinal targeting and characterization of cross-reactivity to multiple AAV serotypes in humansMolecular Therapy - Methods & Clinical DevelopmentSeptember 14, 2023Maura K. Schwartz, Shibi Likhite, Tatyana A. Vetter, Megan C. Baird, Vicki McGovern, Andrea Sierra Delgado, Tom Mendel, Arthur Burghes, Kathrin C. Meyer10 min then followed by 3- to 4-min incubation with True Black Lipofuscin Autofluorescence Quencher (23007, Biotium, Fremont, CA). Slides were washed two times in TBS for 5 min then coverslipped with ProLong Gold Antifade Mountant

(12) An adult clock component links circadian rhythms to pancreatic β-cell maturationbioRxiv : the preprint server for biologyAugust 11, 2023Montalvo AP, Gruskin ZL, Leduc A, Liu M, Gao Z, Ahn JH, Straubhaar JR, Slavov N, Alvarez-Dominguez JR.protected from light. Slides were subsequently washed twice and stained with 1x Trueblack (Biotium; 23007) for 1 minute, followed by washing and incubating with PBS for 5 minutes. For imaging, slides were mounted

(13) Enhanced microglial dynamics and paucity of tau seeding in the amyloid plaque microenvironment contributes to cognitive resilience in Alzheimer’s diseasebioRxivAugust 1, 2023Nur Jury-Garfe, Yanwen You, Pablo Martínez, Javier Redding-Ochoa, Hande Karahan, Travis S. Johnson, Jie Zhang, Jungsu Kim, Juan C. Troncoso, Cristian A. Lasagna-ReevesRT followed by five washes with TBS of 5 min each. Finally, sections were incubated with TrueBlack (23007, Biotium) for 1 min and then washed three times in TBS. Image Analysis Brightfield images were acquired

(14) Prolonged exposure to lung-derived cytokines is associated with inflammatory activation of microglia in patients with COVID-19bioRxivJuly 30, 2023Rogan A Grant, Taylor A. Poor, Lango Sichizya, Estefani Diaz, Joseph I. Bailey, Sahil Soni, Karolina J. Senkow, Xochitl G. Perez-Leonor, Hiam Abdala-Valencia, Ziyan Lu, et al.counterstaining with DAPI, lipofuscin autofluorescence was quenched by treatment with 1x TrueBlack stain (Biotium 23007) in 70% ethanol for 30 seconds followed by 3 washes in PBS. Samples were then counterstained with ACD

(15) Defining the molecular identity and morphology of glia limitans superficialis astrocytes in mouse and humanbioRxivJuly 27, 2023Philip Hasel, Melissa L Cooper, Anne E Marchildon, Uriel A Rufen-Blanchette, Rachel D Kim, Thong C Ma, Un Jung Kang, Moses V Chao, Shane A LiddelowTBS at RT. Lipofuscein autofluorescence was quenched by incubating sections in TrueBlack (Biotium, 23007), sections counterstained with DAPI, covered in mounting media (Fluoromount-G, SouthernBiotech, 0100–01)

(16) PTEN knockout using retrogradely transported AAVs restores locomotor abilities in both acute and chronic spinal cord injurybioRxivJuly 26, 2023Andrew N. Stewart, Reena Kumari, William M. Bailey, Ethan P. Glaser, Gabrielle V. Hammers, Olivia H. Wireman, John C. Genselroom temperature. Spinal cord sections labeled for fluorescent IHC were treated with True Black (0.5x; 23007; Biotium) after antibody labeling to reduce auto-fluorescence caused by lipofuscin accumulation in macrophages. 5.4.3

(17) Neuroimmune characterization of optineurin insufficiency mouse model during ageingScientific reportsJuly 22, 2023Mohovic N, Peradinovic J, Markovinovic A, Cimbro R, Minic Z, Dominovic M, Jakovac H, Nimac J, Rogelj B, Munitic I.(St. Louis, MO, USA) and Tissue-Tek O.C.T. Compound (#4583) from Sakura (Osaka, Japan). TrueBlack (#23007) was from Biotium (San Francisco, CA, USA). Vectashield (#H-1200-10) and ImmEdge Hydrophobic Barrier

(18) Robust induction of functional astrocytes using NGN2 expression in human pluripotent stem cellsiScienceJuly 21, 2023Martin H. Berryer, Matthew Tegtmeyer, Loïc Binan, Vera Valakh, Anna Nathanson, Darina Trendafilova, Ethan Crouse, Jenny A. Klein, Daniel Meyer, Olli Pietiläinen, et al.antibodies, as well as DAPI (1:5000, ThermoFisher Scientific, D1306) and TrueBlack (1:5000, Biotium, 23007). Images were acquired with a high-content screening confocal microscope (Opera Phenix, PerkinElmer,

(19) AAV-GRN partially corrects motor deficits and ALS/FTLD-related pathology in Tmem106b(-/-)Grn(-/-) miceiScienceJuly 21, 2023Feng T, Minevich G, Liu P, Qin HX, Wozniak G, Pham J, Pham K, Korgaonkar A, Kurnellas M, Defranoux NA, et al.collection tube,Sarstedt,Cat# 41.1500.005 TrueBlack Lipofuscin Autofluorescence Quencher,Biotium,Cat# 23007 Odyssey blocking buffer,LI-COR Biosciences,Cat# 927-40000 protease inhibitor,Roche,Cat# 05056489001 Pierce BCA Protein Assay Kit

(20) Multimodal immune cell phenotyping in GI biopsies reveals microbiome-related T cell modulations in human GvHDCell Reports MedicineJuly 18, 2023Sebastian Jarosch, Jan Köhlen, Sakhila Ghimire, Erik Thiele Orberg, Monika Hammel, Doris Gaag, Matthias Evert, Klaus-Peter Janssen, Andreas Hiergeist, André Gessner, et al.acid (EDTA),Carl Roth,Cat#: X986 Sudan Back B,Sigma Aldrich,Cat#: 199664 True Black,Biotium,Cat#: 23007 Dulbecco's phosphate-buffered saline (DPBS),PAN-Biotech,Cat#: P04-36050P Sodium Borohydrate,Sigma

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